October 6, 2022
meso scale discovery assay

The Meso Scale Discovery assay is a robust multiplexing platform for analyzing multiple analytes in complex biological samples. Meso Scale Discovery ELISA combines electrochemiluminescence technology and a multiarray system to bring high-throughput multiplex capabilities to biological assays. A majority of MSD assays are sandwich-based assays consisting of multi-spot plates. Each spot of multi-spot assay plates can be coated with a unique capture antibody. Once samples are incubated with the capture antibodies, Sulfo-tag labeled antibodies detect the binding kinetics. Researchers then apply electricity to the microplate, emitting lights from the Sulfo-Tag labels, which are directly related to the concentration of the analyte in the sample. Such a robust technique has made MSD the primary choice of assays in all drug development stages.

ELISA is a widely used immunoassay for quantifying cytokines and proteins in biological samples. However, they cannot analyze multiple analytes in a single study sample. MSD assays have a broader dynamic range and higher sensitivity than traditional ELISA assays. MSD assays can multiplex up to 10 analytes in a single well. Besides, MSD assays are available in 96-well microplates, where each well can multiplex up to 10 analytes. Hence, sponsors widely use  MSD cytokine assays in crucial strategies for new drug development.

Availability of numerous assays

MSD assays work with a wide range of biological samples, where more than 500 assays are available in panels and reagent kits for bioanalysis. MSD biomarker panels can measure proteins for metabolism, inflammation, immunology, oncology, cardiovascular disease, intracellular signaling, and neurodegeneration. Besides, these assays are compatible with complex biological matrices, including plasma, serum, cell lysate, supernatants, urine, tears, blood spot, CSF, tissue extract, and synovial fluids.

Types of MSD immunoassays

There are four types of MSD assays. They include V-Plex (validated assay), U-Plex (customized multiplex assays), R-Plex (customized assay design), and S-Plex (Extra sensitive assays). Let us dive deep into each of these assay types.

V-Plex assays provide consistency, performance, and reliable results. Besides, these assays are fully validated in different species based on fit-for-purpose approaches. V-Plex assays come in multiplex and singleplex assay variants and are designed for consistent and optimal performance. Moreover, they guarantee lot-to-lot consistency while conducting long-term studies.

The U-Plex assays provide customized options to design singleplex and multiplex immunoassays. The assay couples biotinylated capture antibodies with U-Plex linkers capable of binding with specific spots on the microplate. Besides, these linkers can bind to other target reagents such as peptides, protein, or nucleic acid, thus allowing to work with desired target analytes.

The R-Plex assay also facilitates the development of singleplex and multiplex assays. Each R-Plex assay contains an electrochemiluminescence labeled detection antibody, a biotinylated capture antibody, and a recombinant protein standard. Besides, they have a massive library of emerging biomarkers supported by MSD Multi-array technology.

The S-Plex assays are ideal for detecting analytes present at low concentrations. These are ultrasensitive assay kits for measuring proteins that are generally unmeasurable. They have an optimized detection antibody that increases assay sensitivity, with a detection limit in the femtogram levels.

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